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Physico-Chemical Characterization of the Interaction of Red Fluorescent Protein—DsRed With Thin Silica Layers

Soumbo, Marvine and Pugliara, Alessandro and Monje, Marie-Carmen and Roques, Christine and Despax, Bernard and Bonafos, Caroline and Carles, Robert and Mlayah, Adnen and Makasheva, Kremena Physico-Chemical Characterization of the Interaction of Red Fluorescent Protein—DsRed With Thin Silica Layers. (2016) IEEE Transactions on NanoBioscience, 15 (5). 412-417. ISSN 1536-1241

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Official URL: https://doi.org/10.1109/TNB.2016.2547895

Abstract

The Discosoma recombinant red fluorescent (DsRed) protein is the latest member of the family of fluorescent proteins. It holds great promise for applications in biotechnology and cell biology. However, before being used for rational engineering, knowledge on the behavior of DsRed and the underlying mechanisms relating its structural stability and adsorption properties on solid surfaces is highly demanded. The physico-chemical analysis performed in this study reveals that the interaction of DsRed with SiO2 surfaces does not lead to complete protein denaturation after adsorption and dehydration. Nevertheless, the photoluminescence emission of dehydrated DsRed small droplets was found to be slightly red-shifted, peaking at 590 nm. The measured contact angles of droplets containing different concentration of DsRed proteins determine the interaction as hydrophilic one, however with larger contact angles for larger DsRed concentrations. The DsRed protein behavior is not pH-dependent with respect of the contact angle measurements, in agreement with previously reported studies.

Item Type:Article
HAL Id:hal-01763536
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Institut National des Sciences Appliquées de Toulouse - INSA (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
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Deposited By: Loetitia MOYA
Deposited On:12 Nov 2018 12:27

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