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OTA prevention and detoxification by actinobacterial strains and activated carbon fibers : preliminary results

El Khoury, Rhoda and Choque, Élodie and El Khoury, Anthony and Snini, Selma and Cairns, Robbie and Andriantsiferana, Caroline and Mathieu, Florence OTA prevention and detoxification by actinobacterial strains and activated carbon fibers : preliminary results. (2018) Toxins, 10 (4). 137. ISSN 2072-6651

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Official URL: https://doi.org/10.3390/toxins10040137

Abstract

Ochratoxin A (OTA) is a mycotoxin produced by several species of Aspergillus and Penicillium that contaminate food and feed raw materials. To reduce OTA contamination, we first tested in vitro, actinobacterial strains as potential biocontrol agents and afterward, through a physical decontamination method using activated carbon fibers (ACFs). Actinobacterial strains were screened for their ability to reduce OTA in solid co-culture with A. carbonarius, which is the major OTA-producing species in European vineyards. Four strains showed a high affinity for removing OTA (67%-83%) with no significant effect on fungal growth (<20%). The mechanism of action was first studied by analyzing the expression of OTA cluster genes (acOTApks, acOTAnrps, acOTAhal) by RT-qPCR showing a drastic reduction in all genes (7-15 times). Second, the ability of these strains to degrade OTA was assessed in vitro on ISP2 solid medium supplemented with OTA (100 µg/L). Two strains reduced OTA to undetectable levels. As for the physical method, high adsorption rates were obtained for ACFs at 0.8 g/L with a 50% adsorption of OTA in red wine by AC15 and 52% in grape juice by AC20 within 24 h. These promising methods could be complementarily applied toward reducing OTA contamination in food chains, which promotes food safety and quality.

Item Type:Article
HAL Id:hal-01980263
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Université de Picardie Jules Verne (FRANCE)
Laboratory name:
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Deposited By: Loetitia MOYA
Deposited On:14 Jan 2019 11:01

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