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Supercritical carbon dioxide extraction and fractionation of lipids from freeze-dried microalgae Nannochloropsis oculata and Chlorella vulgaris

Obeid, Sara and Beaufils, Nicolas and Camy, Séverine and Takache, Hosni and Ismail, Ali and Pontalier, Pierre-Yves Supercritical carbon dioxide extraction and fractionation of lipids from freeze-dried microalgae Nannochloropsis oculata and Chlorella vulgaris. (2018) Algal Research, 34. 49-56. ISSN 2211-9264

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Official URL: https://doi.org/10.1016/j.algal.2018.07.003

Abstract

This study deals with the selective extraction of neutral lipids from microalgae. We investigated the consequences of bypassing cell-wall disintegration before supercritical carbon dioxide extraction. Different operating parameters (use of co-solvent, pressure, and time) were tested on freeze-dried Chlorella vulgaris and Nannochloropsis oculata. The solid phase extraction technique (SPE) was used throughout the extraction process to assess variations in the yield of liberated neutral lipids, glycolipids, and phospholipids. Under operating conditions, 97% of neutral lipids were extracted from C. vulgaris using ethanol (10% v/v) as co-solvent. Neutral lipids from N. oculata represented most of the extracts (83%), whereas the proportion of glycolipids and phospholipids did not exceed 12.1% and 5.3%, respectively. Microscopic observation showed that cell wall integrity was maintained during the extraction process.

Item Type:Article
HAL Id:hal-02135624
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Other partners > Lebanese University - LU (LEBANON)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Laboratory name:
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Deposited By: Loetitia MOYA
Deposited On:06 Dec 2018 12:45

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