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Development of a novel quantitative PCR assay as a measurement for the presence of geosmin-producing fungi

Bacha, Nafees and Echarki, Zerif and Mathieu, Florence and Lebrihi, Ahmed Development of a novel quantitative PCR assay as a measurement for the presence of geosmin-producing fungi. (2015) Journal of Applied Microbiology, 118 (5). 1144-1151. ISSN 1364-5072

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Official URL: https://doi.org/10.1111/jam.12747

Abstract

Geosmin‐associated gpe1 gene of Penicillium expansum displayed ≥99% similarity to cytochrome P450 gene of geosmin‐producing P. restrictum, but ≤40% similarities to geosmin biosynthesis, non‐cytochromic gene of Streptomyces avermitilis and cytochrome P450 genes of non‐geosmin‐producing Neotyphodium lolii, Phoma betae and P. paxilli. Serial 10‐fold dilutions of P. expansum's DNA was subjected to a previously reported qPCR assay (Atoui et al. 2007), utilizing gpe1 specific primer pair ‘SNgpe1F/SNgpe1R’. A linear relationship between DNA quantity and Cycle Threshold (Ct), with strong correlative coefficient, was observed. Using the available physico‐chemical method, geosmin was quantified in 188 grape samples. Penicillium spp's DNA was quantified in these samples, utilizing the developed qPCR assay. A strong positive correlation (R2 = 0·97) between Penicillium's DNA and geosmin concentration was observed. Furthermore, <50 ng μl−1 Penicillium's DNA corresponds to geosmin level below the permitted intensity limit i.e. 4, for ‘Flavour Profile Analysis’.

Item Type:Article
HAL Id:hal-02134795
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > University of Peshawar (PAKISTAN)
Other partners > Université de Sherbrooke (CANADA)
Laboratory name:
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Deposited By: Loetitia MOYA
Deposited On:28 Sep 2018 09:20

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