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Bactericidal activity of 3 cutaneous/mucosal antiseptic solutions in the presence of interfering substances: improvement of the NF EN 13727 European Standard ?

Salvatico, Sylvie and Feuillolay, Catherine and Mas, Y. and Verrière, F. and Roques, Christine Bactericidal activity of 3 cutaneous/mucosal antiseptic solutions in the presence of interfering substances: improvement of the NF EN 13727 European Standard ? (2015) Médecine et Maladies Infectieuses, 45 (3). 89-94. ISSN 0399-077X

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Official URL: https://doi.org/10.1016/j.medmal.2015.01.006

Abstract

Objective: there is no standard protocol for the evaluation of antiseptics used for skin and mucous membranes in the presence of interfering substances. Our objective was to suggest trial conditions adapted from the NF EN 13727 standard, for the evaluation of antiseptics used in gynecology and dermatology.Methods: three antiseptic solutions were tested in vitro: a chlorhexidine-benzalkonium (CB) combination, a hexamidine-chlorhexidine-chlorocresol (HCC) combination, and povidone iodine (P). The adaptation of trial conditions to the standard involved choosing dilutions, solvent, and interfering substances. The activity of solutions was assessed on the recommended strains at concentrations of 97% (pure solution), 50%, and 10% (diluted solution), and 1%. A logarithmic reduction ≥ 5 was expected after 60seconds of contact, to meet requirements of bactericidal activity. Results: HCC did not present any bactericidal activity except on P. aeruginosa at a concentration of 97%. P was not bactericidal on E. hirae at any concentration and on S. aureus at 97%. CB had the most homogeneous bactericidal activity with a reduction>5 log on the 4 bacterial strains at concentrations of 97%, 50% and 10%. Conclusion: adapting the NF EN 13727 standard allowed assessing the 3 tested solutions: only CB was bactericidal in dirty conditions. This study proved the possibility of validating antiseptic choice in vitro, in current practice conditions, for adjunctive treatment of skin and mucous membranes disorders, primarily of bacterial origin or with a potential of superinfection.

Item Type:Article
HAL Id:hal-02134801
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Other partners > Laboratoire Innotech International (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > FONdation pour le DEveloppement de la REcherche PHARmaceutique- FONDEREPHAR (FRANCE)
Laboratory name:
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Deposited By: Loetitia MOYA
Deposited On:01 Oct 2018 09:28

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