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Thermoactinomyces khenchelensis sp. nov., a filamentous bacterium isolated from soil sediment of a terrestrial hot spring

Mokrane, Salim and Bouras, Noureddine and Meklat, Atika and Lahoum, Abdelhadi and Zitouni, Abdelghani and Verheecke, Carol and Mathieu, Florence and Schumann, Peter and Spröer, Cathrin and Sabaou, Nasserdine and Klenk, Hans-Peter Thermoactinomyces khenchelensis sp. nov., a filamentous bacterium isolated from soil sediment of a terrestrial hot spring. (2016) Antonie van Leeuwenhoek, 109 (2). 311-317. ISSN 0003-6072

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Official URL: https://doi.org/10.1007/s10482-015-0634-9

Abstract

A novel thermophilic filamentous bacterium, designated strain T36T, was isolated from soil sediment sample from a hot spring source collected in Khenchela province, Algeria. Strain T36T was identified as a member of the genus Thermoactinomyces by a polyphasic approach. Strain T36T was observed to form white aerial mycelium and non-coloured to pale yellow substrate mycelium, both producing endospores, sessile or borne by short sporophores. The optimum growth temperature and pH were found to be 37–55 °C and 7.0–9.0, respectively and the optimum NaCl concentration for growth was found to be 0–7 % (w/v). The diagnostic diamino acid in the cell wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinone of strain T36T was identified as MK-7 (H0). The major fatty acids were found to be iso-C15:0 and iso-C17:0. The phospholipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphoglycolipid. The chemotaxonomic properties of strain T36T are consistent with those shared by members of the genus Thermoactinomyces. 16S rRNA gene sequence analysis indicated that the sequence similarities between strain T36T and Thermoactinomyces species with validly published names were less than 98 %. Based on the combined genotypic and phenotypic evidence, it is proposed that strain T36T should be classified as representative of a novel species, for which the name Thermoactinomyces khenchelensis sp. nov. is proposed. The type strain is T36T (=DSM 45951T = CECT 8579T).

Item Type:Article
HAL Id:hal-02134872
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Leibniz-Institut Deutsche Sammlung von Mikroorganismen und Zelllkulturen GmBH - DSMZ (GERMANY)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Université de Ghardaïa (ALGERIA)
Other partners > Newcastle University (UNITED KINGDOM)
Other partners > Université Saad Dahleb (ALGERIE)
Laboratory name:
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Deposited By: Loetitia MOYA
Deposited On:25 Oct 2018 15:03

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