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Actinomadura adrarensis sp. nov., an actinobacterium isolated from Saharan soil

Lahoum, Abdelhadi and Bouras, Noureddine and Verheecke, Carol and Mathieu, Florence and Schumann, Peter and Spröer, Cathrin and Klenk, Hans-Peter and Sabaou, Nasserdine Actinomadura adrarensis sp. nov., an actinobacterium isolated from Saharan soil. (2016) International Journal of Systematic and Evolutionary Microbiology, 66 (7). 2724-2729. ISSN 1466-5026

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Official URL: https://doi.org/10.1099/ijsem.0.001114

Abstract

A novel actinobacterial strain, designated ACD12T, was isolated from a Saharan soil sample collected from Adrar province, southern Algeria. A polyphasic study was carried out to establish the taxonomic position of this strain. Strain ACD12T was observed to form extensively branched substrate mycelia. Aerial mycelium was absent or was weakly produced on all media tested, while spore chains were short with a hooked and irregular spiral form (2–3 turns). The dominant diaminopimelic acid isomer in the cell wall was meso-diaminopimelic acid. Glucose, ribose, galactose, mannose and madurose occured in whole-cell hydrolysates. The major phospholipid was diphosphatidylglycerol and phosphatidylinositol. The predominant menaquinone was MK-9(H6). The fatty acid profile was characterized by the presence of C16 : 0, C17 : 0, C15 : 0, C18 : 0, C18 : 1 cis9 and iso-C16 : 0. Results of 16S rRNA gene sequence comparisons revealed that strain ACD12T shared the highest degree of 16S rRNA gene sequence similarity with Actinomadura sputi DSM 45233T (98.3 %) and Actinomadura hallensis DSM 45043T (97.8 %). All tree-making algorithms used also supported strain ACD12T forming a distinct clade with its most closely related species. In addition, DNA–DNA hybridization indicated only 39.8 % relatedness with A. sputi DSM 45233T and 18.7 % relatedness with A. hallensis DSM 45043T. The combined phenotypic and genotypic data show that the novel isolate represents a novel species of the genus Actinomadura , for which the name Actinomadura adrarensis sp. nov., is proposed, with the type strain ACD12T (=DSM 46745T =CECT 8842T).

Item Type:Article
HAL Id:hal-01907335
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Leibniz-Institut Deutsche Sammlung von Mikroorganismen und Zelllkulturen GmBH - DSMZ (GERMANY)
Other partners > Ecole normale supérieure de Kouba (ALGERIA)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Université de Ghardaïa (ALGERIA)
Other partners > Newcastle University (UNITED KINGDOM)
Laboratory name:
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Deposited By: Loetitia MOYA
Deposited On:26 Oct 2018 09:53

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