OATAO - Open Archive Toulouse Archive Ouverte Open Access Week

Discerning different and opposite effects of hydrogenase on the corrosion of mild steel in the presence of phosphate species

Mehanna, Maha and Rouvre, Ingrid and Délia, Marie-Line and Féron, Damien and Bergel, Alain and Basséguy, Régine Discerning different and opposite effects of hydrogenase on the corrosion of mild steel in the presence of phosphate species. (2016) Bioelectrochemistry, 111. 31-40. ISSN 1567-5394

[img]
Preview
(Document in English)

PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
8MB

Official URL: https://doi.org/10.1016/j.bioelechem.2016.04.005

Abstract

Mild steel coupons were exposed to hydrogenase in a 10 mM phosphate solution. Control coupons were covered by a layer of vivianite. The injection of hydrogenase caused a fast increase in the open circuit potential; this increase depended on the amount of hydrogenase injected and increased from 8 mV for 30 μL hydrogenase to 63 mV for 80 μL. The presence of enzyme resulted in a thicker deposit: high amounts induced the accumulation of corrosion products. Hydrogenase that was deactivated by air revealed a protective effect: non-degradation was observed. In contrast, hydrogenase that was denatured by heat provoked an important deposit of corrosion products with a heterogeneous, cracked structure. The study showed that the action of hydrogenase is not linked to its regular enzymatic activity but to a balance between the protective effect of its protein shell and the electrochemical action of its iron-sulphur clusters. Depending on the operating conditions, hydrogenase can either enhance or mitigate the formation of a corrosion layer on mild steel.

Item Type:Article
HAL Id:hal-01907323
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Commissariat à l'Energie Atomique et aux énergies alternatives - CEA (FRANCE)
French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Other partners > Université Paris-Saclay (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Laboratory name:
Statistics:download
Deposited By: Loetitia MOYA
Deposited On:23 Oct 2018 12:34

Repository Staff Only: item control page