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Use of a continuous-flow bioreactor to evaluate nitrate reduction rate of Halomonas desiderata in cementitious environment relevant to nuclear waste deep repository

Rafrafi, Yan and Durban, Nadège and Bertron, Alexandra and Albrecht, Achim and Robinet, Jean-Charles and Erable, Benjamin Use of a continuous-flow bioreactor to evaluate nitrate reduction rate of Halomonas desiderata in cementitious environment relevant to nuclear waste deep repository. (2017) Biochemical Engineering Journal, 125. 161-170. ISSN 1369-703X

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Official URL: http://doi.org/10.1016/j.bej.2017.05.016

Abstract

The redox level of repositories can influence the mobility of the waste components stored in them (i.e. radionuclides) and thus the related safety assessments. Microbial activity is known to impact the control of redox reactions, the mechanisms and kinetics of which must be evaluated. This study investigates the denitrification rates of a model bacterium Halomonas desiderata (Hd) in cementitious environment with alkaline and anoxic conditions comparable to those found in radioactive waste repository cells. The growth and the total oxidized nitrogen (TON) reduction rates of Hd was determined in a continuous bioreactor with several feeding solutions with or without solid cement paste. Temporary nitrite accumulation and reduced denitrification rates are correlated with diminished bacterial growth. When the system was fed by optimal culture medium supplemented with acetate and nitrate, the TON reduction rates varied between 0.082 mM TON/h and 0.063 mM TON/h, depending on whether solid cement paste was present in the reactor or not. When the culture medium was replaced with pure cement leachate, the reaction rates increased to 0.137 mM TON/h with solid cement paste and dropped to 0.023 mM TON/h without. In these conditions at pH 10, solid cement paste had no negative influence on Hd activity.

Item Type:Article
HAL Id:hal-01845542
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Agence Nationale pour la Gestion des Déchets Radioactifs - ANDRA (FRANCE)
French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Institut National des Sciences Appliquées de Toulouse - INSA (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
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Deposited By: Loetitia MOYA
Deposited On:20 Jul 2018 12:35

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