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EPR Spectroelectrochemical Investigation of Guanine Radical Formation and Environment Effects

Ribaut, Clotilde and Bordeau, Guillaume and Perio, Pierre and Reybier, Karine and Sartor, Valérie and Reynes, Olivier and Fabre, Paul-Louis and Chouini-Lalanne, Nadia EPR Spectroelectrochemical Investigation of Guanine Radical Formation and Environment Effects. (2014) Journal of Physical Chemistry B, 118 (9). 2360-2365. ISSN 1520-6106

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Official URL: https://doi.org/10.1021/jp500952q

Abstract

Guanine radical detection was carried out by a new convenient and efficient method coupling electron paramagnetic resonance spectroscopy and indirect electrooxidation of guanine in different biological environments, from the free nucleotide to several types of DNA substrates. Compared to the widely used photoirradiation method, this method appeared more selective in the choice of the electrochemical mediator. Carried out in presence of a ruthenium mediator and PBN as spin trap, this method revealed two types of EPR spectra depending of the environment of the guanine radical. Both EPR spectra show the trapping of the neutral guanine radical G(−H)• obtained after fast deprotonation of the radical cation G•+. However, they differ by the atom where the trapped radical is centered. This difference highlights the structural dependency of the environment on the nature of the radical formed. This work gave the evidence of an innovative method to detect in situ the guanine radical.

Item Type:Article
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
French research institutions > Institut de Recherche pour le Développement - IRD (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
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Deposited By: Loetitia MOYA
Deposited On:11 Oct 2018 11:12

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