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Cloud Point Extraction of α-Amino Acids

Ghouas, Halima and Haddou, Boumediene and Kameche, Mostéfa and Louhibi, Lotfi and Derriche, Zoubir and Canselier, Jean-Paul and Gourdon, Christophe Cloud Point Extraction of α-Amino Acids. (2014) Separation Science and Technology, 49 (14). 2142-2150. ISSN 0149-6395

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Official URL: https://doi.org/10.1080/01496395.2014.919322

Abstract

Cloud point extraction with a polyethoxylated alcohol (Oxo-C10E4) is used to separate five α-amino acids: alanine, valine, leucine, isoleucine, and phenylalanine (0.75 wt.% in water), and their extraction efficiencies are compared. The variables affecting phase separation and extraction (wt.% surfactant and equilibrium temperature) are optimized using experimental design. The four responses are: percentage of solute extracted (E), residual concentrations of solute (amino acid) and surfactant in the dilute phase, and volume fraction of coacervate at equilibrium. E increases with surfactant concentration and amino acid hydrophobicity in the following order: alanin < valin < leucin < isoleucine < phenylalanine, with respective maximum values: 73, 74, 76, 78.5, and 95%, and decreases with a temperature rise. It also makes sense that aspartic and glutamic acids, much more hydrophilic, are poorly extracted (E ˜ 10%). The trend observed is consistent with water/n-octanol partition coefficient (Log P) of amino acids in pure water. A more detailed study is presented for alanine and phenylalanine. Addition of sodium sulphate or cetylammonium bromide greatly raises extraction rates.

Item Type:Article
HAL Id:hal-01924716
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Université des Sciences et de la Technologie Mohamed Boudiaf d'Oran - USTO (ALGERIA)
Laboratory name:
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Deposited By: Loetitia MOYA
Deposited On:16 Nov 2018 10:14

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