OATAO - Open Archive Toulouse Archive Ouverte Open Access Week

Graphene oxide impairs the pollen performance of Nicotiana tabacum and Corylus avellana suggesting potential negative effects on the sexual reproduction of seed plants

Candotto Carniel, Fabio and Gorelli, Davide and Flahaut, Emmanuel and Fortuna, Lorenzo and Del Casino, Cecilia and Cai, Giampiero and Nepi, Massimo and Prato, Maurizio and Tretiach, Mauro Graphene oxide impairs the pollen performance of Nicotiana tabacum and Corylus avellana suggesting potential negative effects on the sexual reproduction of seed plants. (2018) Environmental Science Nano. ISSN 2051-8153

[img]
Preview
(Document in English)

PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
1MB

Official URL: http://doi.org/10.1039/c8en00052b

Abstract

The production of graphene based materials (GBMs) is steadily increasing but the effects of the possible release of GBMs in the environment are far from being understood. Graphene oxide (GO) is among the most active GBMs and it causes widely varying effects on the vegetative body of seed plants. However, nothing is known yet about its potential effects on the reproductive process. This study addresses the effects of GO on pollen germination and pollen tube elongation in the model species Nicotiana tabacum and in the non-model species Corylus avellana. In vitro germination experiments were conducted without or with GO (control and treated samples, respectively) at concentrations of 25, 50 and 100 μg mL−1. Pollen germination and tube elongation were affected at GO concentrations ≥50 μg mL−1, decreasing by 20% and 19% in N. tabacum and by 68% and 58% in C. avellana, respectively. GO did not affect the viability of N. tabacum pollen, but doubled the frequency of bent tubes. Microscopy observations of pollen tubes exposed to a cellpermeant, dual-excitation ratiometric pH indicator revealed that GO affected the intracellular pH homeostasis. Further germination experiments on C. avellana conducted by inverting the pH conditions of the control and treated (100 μg GO mL−1) samples demonstrated that the main factor influencing the pollen performance is the acidic properties of GO. This might affect the reproductive process of numerous seed plants thus being relevant from an environmental point of view.

Item Type:Article
Additional Information:Thanks to ASC editor. The original PDF of the article can be found at http://pubs.rsc.org/en/Content/ArticleLanding/2018/EN/C8EN00052B
HAL Id:hal-01811152
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Centro de Investigación Cooperativa en Biomateriales - CIC biomaGUNE (SPAIN)
Other partners > Università degli Studi di Siena - UNISI (ITALY)
Other partners > Università degli studi di Trieste (ITALY)
Laboratory name:
Statistics:download
Deposited By: cirimat webmestre
Deposited On:08 Jun 2018 13:49

Repository Staff Only: item control page