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New insights into Legionella pneumophila biofilm regulation by c-di-GMP signaling

Pécastaings, Sophie and Allombert, Julie and Lajoie Halova, Barbora and Doublet, Patricia and Roques, Christine and Vianney, Anne New insights into Legionella pneumophila biofilm regulation by c-di-GMP signaling. (2016) Biofouling, 12 (8). 935-948. ISSN 0892-7014

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Official URL: https://doi.org/10.1080/08927014.2016.1212988

Abstract

The waterborne pathogen Legionella pneumophila grows as a biofilm, freely or inside amoebae. Cyclic-di-GMP (c-di-GMP), a bacterial second messenger frequently implicated in biofilm formation, is synthesized and degraded by diguanylate cyclases (DGCs) and phosphodiesterases (PDEs), respectively. To characterize the c-di-GMP-metabolizing enzymes involved in L. pneumophila biofilm regulation, the consequences on biofilm formation and the c-di-GMP concentration of each corresponding gene inactivation were assessed in the Lens strain. The results showed that one DGC and two PDEs enhance different aspects of biofilm formation, while two proteins with dual activity (DGC/PDE) inhibit biofilm growth. Surprisingly, only two mutants exhibited a change in global c-di-GMP concentration. This study highlights that specific c-di-GMP pathways control L. pneumophila biofilm formation, most likely via temporary and/or local modulation of c-di-GMP concentration. Furthermore, Lpl1054 DGC is required to enable the formation a dense biofilm in response to nitric oxide, a signal for biofilm dispersion in many other species.

Item Type:Article
Additional Information:Thanks to Taylor & Francis editor. The original PDF of the article can be found at Biofouling website : https://www.tandfonline.com/doi/full/10.1080/08927014.2016.1212988
HAL Id:hal-01757057
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Ecole Normale Supérieure de Lyon - ENS de Lyon (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
French research institutions > Institut National de la Santé et de la Recherche Médicale - INSERM (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Université de Lyon - UDL (FRANCE)
Laboratory name:
Funders:
Ministère de l’Enseignement et de la Recherche. - ANSES (French Agency for Food, Environmental and Occupational Health & Safety
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Deposited By: Sophie Pecastaings
Deposited On:03 Apr 2018 10:48

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