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In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains

Pignolet, Béatrice and Duteyrat, Jean-Luc and Allemandou, Aude and Gelfi, Jacqueline and Foucras, Gilles and Bertagnoli, Stéphane In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains. (2007) Virology Journal, vol. 4 (n° 94). ISSN 1743-422X

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Official URL: http://www.virologyj.com/content/4/1/94

Abstract

Myxoma virus (MYXV), a leporide-specific poxvirus, represents an attractive candidate for the generation of safe, non-replicative vaccine vector for non-host species. However, there is very little information concerning infection of non-laboratory animals species cells with MYXV. In this study, we investigated interactions between bovine cells and respectively a wild type strain (T1) and a vaccinal strain (SG33) of MYXV. We showed that bovine KOP-R, BT and MDBK cell lines do not support MYXV production. Electron microscopy observations of BT-infected cells revealed the low efficiency of viral entry and the production of defective virions. In addition, infection of bovine peripheral blood mononuclear cells (PBMC) occurred at a very low level, even following nonspecific activation, and was always abortive. We did not observe significant differences between the wild type strain and the vaccinal strain of MYXV, indicating that SG33 could be used for new bovine vaccination strategies.

Item Type:Article
Additional Information:Original PDF is authorized. This article of Virology Journal is online on Biomed Central website : http://www.virologyj.com/content/4/1/94
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut National de la Recherche Agronomique - INRA
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT
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Deposited By:Mathieu Andro

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