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Switching on microglia with electro-conductive multi walled carbon nanotubes

Fiorito, Silvana and Russier, Julie and Salemme, Adele and Soligo, Marzia and Manni, Luigi and Krasnowska, Ewa and Bonnamy, Sylvie and Flahaut, Emmanuel and Serafino, Annalucia and Togna, Giuseppina Ines and Marlier, Lionel N.J.L. and Togna, Anna Rita Switching on microglia with electro-conductive multi walled carbon nanotubes. (2018) Carbon, 129. 572-584. ISSN 0008-6223

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Official URL: http://dx.doi.org/10.1016/j.carbon.2017.12.069

Abstract

We explored the mechanisms underlying microglia cell-carbon nanotube interactions in order to investigate whether electrical properties of Carbon-Nanotubes (CNTs) could affect microglia brain cells function and phenotype. We analyzed the effects induced by highly electro-conductive Multi-Walled-Carbon-Nanotubes (a-MWCNTs), on microglia cells from rat brain cortex and compared the results with those obtained with as prepared not conductive MWCNTs (MWCNTs) and redox-active Double-Walled-Carbon-Nanotubes (DWCNTs). Cell viability and CNT capacity to stimulate the release of nitric oxide (NO), pro-inflammatory (IL-1b, TNF-a) and anti-inflammatory (IL-10, TGF-b1) cytokines and neurotrophic factors (mNGF) were assessed. Electro-conductive MWCNTs, besides not being cytotoxic, were shown to stimulate, at 24 h cell exposure, classical "M100 microglia activation phenotype, increasing significantly the release of the main pro-inflammatory cytokines. Conversely, after 48 h cell exposure, they induced the transition from classical "M100 to alternative "M200 microglia phenotype, supported by anti-inflammatory cytokines and neuroprotective factor mNGF release. The analysis of cell morphology change, by tubulin and CD-206 þ labelling showed that M2 phenotype was much more expressed at 48 h in cells exposed to a-MWCNTs than in untreated cells. Our data suggest that the intrinsic electrical properties of CNTs could be exploited to modulate microglia phenotype and function stimulating microglia anti-inflammatory potential.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at : https://www.sciencedirect.com/science/article/pii/S0008622317313027
HAL Id:hal-01684006
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Consiglio Nazionale delle Ricerche - CNR (ITALY)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Other partners > Università di Roma - SAPIENZA (ITALY)
Other partners > Université de Strasbourg - UNISTRA (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Université d'Orléans (FRANCE)
Laboratory name:
Funders:
Federica Andreola - Institute of Translational Pharmacology
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Deposited By: cirimat webmestre
Deposited On:15 Jan 2018 08:59

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