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Competition between covalent and non-covalent grafting of fluorescein isothiocyanate on double-walled carbon nanotubes: a quantitative approach

Lorne, Thomas and Jiménez-Ruiz, Mónica and Rols, Stéphane and Escudier, Jean-Marc and Rubio-Zuazo, Juan and Zbiri, Mohamed and Galibert, Anne-Marie and Soula, Brigitte and Flahaut, Emmanuel Competition between covalent and non-covalent grafting of fluorescein isothiocyanate on double-walled carbon nanotubes: a quantitative approach. (2017) Carbon, 123. 735-743. ISSN 0008-6223

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Official URL: http://doi.org/10.1016/j.carbon.2017.07.070

Abstract

The functionalization of carbon nanotubes with fluorescent molecules is a standard procedure in many toxicity studies aiming at knowing their distribution within cells or whole organisms. Nevertheless, there is a lack of knowledge concerning the efficiency of the grafting processes, and more specifically concerning the question of the competition between covalent and non-covalent grafting. In this work, we investigated the grafting process of the fluorescein isothiocyanate (FITC) onto double-walled carbon nanotubes (DWNTs) using X-ray photoelectron spectroscopy, inelastic neutron scattering spectroscopy and computational simulations.We demonstrated that both covalent and non-covalent grafting occurred during the functionalization with the FITC. Moreover, we showed that a significant fraction of the fluorophore remained simply adsorbed onto the DWNTs despite thorough washing steps, which raises concerning questions about the use of this fluorophore in some toxicity studies and its possible ability to mislead their conclusions.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The original PDF of the article can be found at https://www.sciencedirect.com/science/article/pii/S0008622317307522
HAL Id:hal-01813417
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > European Synchrotron Radiation Facility - ESRF (FRANCE)
Other partners > Institut Laue-Langevin - ILL (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
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Funders:
Université Paul Sabatier (UPS) - Institut Laue Langevin (ILL)
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Deposited By: cirimat webmestre
Deposited On:12 Jun 2018 11:04

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