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The Pseudomonas fluorescens siderophore pyoverdine weakens arabidopsis thaliana defense in favor of growth in iron-deficient conditions

Trapet, Pauline and Avoscan, Laure and Klinguer, Agnès and Pateyron, Stéphanie and Citerne, Sylvie and Chervin, Christian and Mazurier, Sylvie and Lemanceau, Philippe and Wendehenne, David and Besson-Bard, Angélique The Pseudomonas fluorescens siderophore pyoverdine weakens arabidopsis thaliana defense in favor of growth in iron-deficient conditions. (2016) plant physiology, 171 (1). 675-693. ISSN 1532-2548

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Official URL: http://dx.doi.org/10.1104/pp.15.01537

Abstract

Pyoverdines are siderophores synthesized by fluorescent Pseudomonas spp. Under iron-limiting conditions, these high-affinity ferric iron chelators are excreted by bacteria in the soil to acquire iron. Pyoverdines produced by beneficial Pseudomonas spp. ameliorate plant growth. Here, we investigate the physiological incidence and mode of action of pyoverdine from Pseudomonas fluorescens C7R12 on Arabidopsis (Arabidopsis thaliana) plants grown under iron-sufficient or iron-deficient conditions. Pyoverdine was provided to the medium in its iron-free structure (apo-pyoverdine), thus mimicking a situation in which it is produced by bacteria. Remarkably, apo-pyoverdine abolished the iron-deficiency phenotype and restored the growth of plants maintained in the iron-deprived medium. In contrast to a P. fluorescens C7R12 strain impaired in apo-pyoverdine production, the wild-type C7R12 reduced the accumulation of anthocyanins in plants grown in iron-deficient conditions. Under this condition, apo-pyoverdine modulated the expression of around 2,000 genes. Notably, apo-pyoverdine positively regulated the expression of genes related to development and iron acquisition/redistribution while it repressed the expression of defense-related genes. Accordingly, the growth-promoting effect of apo-pyoverdine in plants grown under iron-deficient conditions was impaired in iron-regulated transporter1 and ferric chelate reductase2 knockout mutants and was prioritized over immunity, as highlighted by an increased susceptibility to Botrytis cinerea This process was accompanied by an overexpression of the transcription factor HBI1, a key node for the cross talk between growth and immunity. This study reveals an unprecedented mode of action of pyoverdine in Arabidopsis and demonstrates that its incidence on physiological traits depends on the plant iron status.

Item Type:Article
Additional Information:Thanks to The American Society of Plant Biologists editor. The definitive version is available at : http://www.plantphysiol.org/content/171/1/675
HAL Id:hal-01636086
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > AgroParisTech (FRANCE)
Other partners > Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement - AgroSup Dijon (FRANCE)
French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
French research institutions > Institut des Sciences des Plantes de Paris-Saclay - IPS2 (FRANCE)
Other partners > Université de Paris Diderot - Paris 7 (FRANCE)
Other partners > Université de Bourgogne - UB (FRANCE)
Other partners > Université d'Evry - Val-d'Essonne (FRANCE)
Other partners > Université Paris-Sud 11 (FRANCE)
Laboratory name:
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Deposited By: Christian CHERVIN
Deposited On:16 Nov 2017 09:01

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