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Correlations between effective permeability and marrow contact channels surface of vertebral endplates

Laffosse, Jean-Michel and Accadbled, Franck and Molinier, François and Bonnevialle, Nicolas and Sales de Gauzy, Jérôme and Swider, Pascal Correlations between effective permeability and marrow contact channels surface of vertebral endplates. (2010) Journal of Orthopaedic Research, 28 (9). 1229-1234. ISSN 0736-0266

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Official URL: http://dx.doi.org/10.1002/jor.21137

Abstract

Homeostasis of the intervertebral disc relies on nutrient supply and waste clearance through the dense capillary network that is in contact with the cartilage endplate (CEP). We developed a micro-computerized tomography (micro-CT) method to quantify the marrow contact channel surface (MCCS) with the CEP and to validate the hypothesis according to which MCCS was correlated to the effective permeability of the vertebral endplate (VEP) and influenced by the mechanical stimuli. The influence of compression loading on local vascularization was investigated. Six 4-week-old skeletally immature pigs were instrumented with left pedicle screws and rod at both T5-T6 and L1-L2 levels to create asymmetrical spine tethers. After 3 months of growth, three cylindrical specimens of the VEP (one central and two lateral right and left) were obtained from both the instrumented and the control levels. We used a previously validated method for measuring permeability. Micro-CT analysis (resolution 12 mu m) yielded a gray-scale 2D-image of the discal end of each specimen converted into a binary 2D-image to derive the MCCS. Correlations between MCCS and effective permeability were assessed. Effective permeability and MCCS were significantly decreased compared to the control group especially on the tethered side (-41..5%, p = 0.004 and -52.5%, p = 0.0009, respectively). Correlations were significant and showed maximal value (r(2) = 0.430, p < 0.0001) on the tethered side involving maximal compressive loadings. Mechanical stimuli, due to unbalanced growth, altered the vascularization and the convective properties of the CEP. The cascade of mechanobiological events should offer perspectives for research on disc degeneration and attempted treatment. (C) 2010 Orthopaedic Research Society.

Item Type:Article
Additional Information:Thanks to Wiley editor. The definitive version is available at http://onlinelibrary.wiley.com/ The original PDF of the article can be found at http://onlinelibrary.wiley.com/doi/10.1002/jor.21137/epdf
HAL Id:hal-01591232
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Centre Hospitalier Universitaire de Toulouse - CHU Toulouse (FRANCE)
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Deposited By: Pascal SWIDER
Deposited On:21 Sep 2017 08:17

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