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Role of ethylene in the biosynthetic pathway of aliphatic ester aroma volatiles in Charentais Cantaloupe melons

Flores, Francisco B. and El-Yahyaoui, Fikri and De Billerbeck, G. and Romojaro, Felix and Latché, Alain and Bouzayen, Mondher and Pech, Jean-Claude and Ambid, C. Role of ethylene in the biosynthetic pathway of aliphatic ester aroma volatiles in Charentais Cantaloupe melons. (2002) Journal of Experimental Botany, vol. 5 (n° 367). pp. 201-206. ISSN 0022-0957

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Official URL: http://jxb.oxfordjournals.org/cgi/content/abstract/53/367/201?ck=nck

Abstract

Compared to other melon types, Cantaloupe Charentais melons are highly aromatic with a major contribution to the aroma being made by aliphatic and branched esters. Using a transgenic line in which the synthesis of the plant hormone ethylene has been considerably lowered by antisense ACC oxidase mRNA (AS), the aliphatic ester pathway steps at which ethylene exerts its regulatory role were found. The data show that the production of aliphatic esters such as hexyl and butyl acetate was blocked in AS fruit and could be reversed by ethylene. Using fruit discs incubated in the presence of various precursors, the steps at which ester formation was inhibited in AS fruit was shown to be the reduction of fatty acids and aldehydes, the last step of acetyl transfer to alcohols being unaffected. However, treating AS fruit with the ethylene antagonist 1-methylcyclopropene resulted in about 50% inhibition of acetyl transfer activity, indicating that this portion of activity was ethylene-dependent and this was supported by the low residual ethylene concentration of AS fruit discs (around 2 µl l-1). In conclusion, the reduction of fatty acids and aldehydes appears essentially to be ethylene-dependent, whilst the last step of alcohol acetylation has ethylene-dependent and ethylene-independent components, probably corresponding to differentially regulated alcohol acetyltransferases.

Item Type:Article
Additional Information:The definitive version is available at http://jxb.oxfordjournals.org The original PDF of the article can be found at Journal of Experimental Botany website : http://jxb.oxfordjournals.org/cgi/content/abstract/53/367/201?ck=nck
Audience (journal):International peer-reviewed journal
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Institution:French research institutions > Institut National de la Recherche Agronomique - INRA
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
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Deposited By: Dabernat Séverine

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