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Conformational and thermal characterization of left ventricle remodeling post-myocardial infarction

Samouillan, Valérie and Revuelta-López, Elena and Soler-Botija, C. and Dandurand, Jany and Benitez-Amaro, Aleyda and Nasarre, Laura and De Gonzalo-Calvo, D and Bayes-Genis, A and Lacabanne, Colette and Llorente-Cortés, Vicenta Conformational and thermal characterization of left ventricle remodeling post-myocardial infarction. (2017) Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1863 (6). 1500-1509. ISSN 0925-4439

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Official URL: http://dx.doi.org/10.1016/j.bbadis.2017.02.025

Abstract

Adverse cardiac remodeling after myocardial infarction (MI) causes impaired ventricular function and heart failure. Histopathological characterization is commonly used to detect the location, size and shape of MI sites. However, the information about chemical composition, physical structure and molecular mobility of peri- and infarct zones post-MI is rather limited. The main objective of this work was to explore the spatiotemporal biochemical and biophysical alterations of key cardiac components post-MI. The FTIR spectra of healthy and remote myocardial tissue shows amides A, I, II and III associated with proteins in freeze-died tissue as major absorptions bands. In infarcted myocardium, the spectrum of these main absorptions was deeply altered. FITR evidenced an increase of the amide A band and the distinct feature of the collagen specific absorption band at 1338cm-1 in the infarct area at 21days post-MI. At 21days post-MI, it also appears an important shift of amide I from 1646cm-1 to 1637cm-1 that suggests the predominance of the triple helical conformation in the proteins. The new spectra bands also indicate an increase in proteoglycans, residues of carbohydrates in proteins and polysaccharides in ischemic areas. Thermal analysis indicates a deep increase of unfreezable water/freezable water in peri- and infarcted tissues. In infarcted tissue is evidenced the impairment of myofibrillar proteins thermal profile and the emergence of a new structure. In conclusion, our results indicate a profound evolution of protein secondary structures in association with collagen deposition and reorganization of water involved in the scar maturation of peri- and infarct zones post-MI.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The original PDF of the article can be found at http://www.sciencedirect.com/science/article/pii/S0925443917300741?via%3Dihub
HAL Id:hal-01565042
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Consejo Superior de Investigaciones Científicas - CSIC (SPAIN)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Universitat Autònoma de Barcelona - UAB (SPAIN)
Other partners > Hospital Universitari Germans Trias i Pujol - HUGTP (SPAIN)
Other partners > Hospital de la Santa Creu i Sant Pau (SPAIN)
Other partners > Instituto de Salud Carlos III - ISC (SPAIN)
Other partners > Institut d'Invertigacio en Ciències de la Salut Germans Trias i Pujol - IGTP (SPAIN)
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Deposited By: cirimat webmestre
Deposited On:19 Jul 2017 12:10

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