OATAO - Open Archive Toulouse Archive Ouverte Open Access Week

Purification and Characterization of a NADPH-Dependent Aldehyde Reductase from Mung Bean That Detoxifies Eutypine, a Toxin from Eutypa lata1

Colrat, Ségolène and Latché, Alain and Guis, Monique and Pech, Jean-Claude and Bouzayen, Mondher and Fallot, Jean and Roustan, Jean-Paul Purification and Characterization of a NADPH-Dependent Aldehyde Reductase from Mung Bean That Detoxifies Eutypine, a Toxin from Eutypa lata1. (1999) Plant Physiology, vol. 1 . pp. 621-626. ISSN 0032-0889

[img] (Document in English)

PDF (Author's version) - Depositor and staff only - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
227kB

Official URL: http://www.plantphysiol.org/cgi/content/abstract/119/2/621

Abstract

Eutypine (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzaldehyde) is a toxin produced by Eutypa lata, the causal agent of eutypa dieback in the grapevine (Vitis vinifera). Eutypine is enzymatically converted by numerous plant tissues into eutypinol (4-hydroxy-3- [3-methyl-3-butene-1-ynyl] benzyl alcohol), a metabolite that is nontoxic to grapevine. We report a four-step procedure for the purification to apparent electrophoretic homogeneity of a eutypinereducing enzyme (ERE) from etiolated mung bean (Vigna radiata) hypocotyls. The purified protein is a monomer of 36 kD, uses NADPH as a cofactor, and exhibits a Km value of 6.3 mM for eutypine and a high affinity for 3- and 4-nitro-benzaldehyde. The enzyme failed to catalyze the reverse reaction using eutypinol as a substrate. ERE detoxifies eutypine efficiently over a pH range from 6.2 to 7.5. These data strongly suggest that ERE is an aldehyde reductase that could probably be classified into the aldo-keto reductase superfamily. We discuss the possible role of this enzyme in eutypine detoxification.

Item Type:Article
Additional Information:The definitive version is available at http://www.plantphysiol.org/ The original PDF of the article can be found at Plant Physiology website : http://www.plantphysiol.org/cgi/content/abstract/119/2/621
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut National de la Recherche Agronomique - INRA
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Laboratory name:
Statistics:download
Deposited By: Dabernat Séverine
Deposited On:09 Dec 2008 12:16

Repository Staff Only: item control page