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Constitutive expression of Vr-ERE gene in transformed grapevines confers enhanced resistance to eutypine, a toxin from Eutypa lata

Legrand, Valérie and Dalmayrac, Sylvie and Latché, Alain and Pech, Jean-Claude and Bouzayen, Mondher and Fallot, Jean and Torregrosa, Laurent and Bouquet, Alain and Roustan, Jean-Paul Constitutive expression of Vr-ERE gene in transformed grapevines confers enhanced resistance to eutypine, a toxin from Eutypa lata. (2003) Plant Science, vol. 1 (n°5). pp. 809-814. ISSN 0168-9452

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Official URL: http://dx.doi.org/10.1016/S0168-9452(03)00069-4

Abstract

Eutypine (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzaldehyde) is a toxin produced by Eutypa lata , the causal agent of eutypa dieback of grapevines. Recently, a eutypine detoxifying gene (Vr-ERE) encoding an NADPH-dependent aldehyde reductase which converts eutypine into the corresponding alcohol, eutypinol, a non-toxic form of the toxin, has been cloned. We report here the obtention of transgenic plants from grapevine rootstock 110 Richter Vitis berlandieri/V. rupestris that express the Vr-ERE gene. The over-expression of Vr-ERE gene in grapevine tissues increases their detoxification capacity. The growth and development of the transgenic plants cultured in vitro exhibiting a high Vr-ERE activity were not affected by the presence of 300 mM of toxin whereas the growth of untransformed control plants was highly inhibited. These results suggest that the Vr-ERE gene is an efficient candidate to confer resistance to the toxin and opens new opportunities to study the role of the toxin in the development of the disease.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Plant Science website : http://www.sciencedirect.com/science/journal/01689452
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut National de la Recherche Agronomique - INRA
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
French research institutions > Institut de Recherche pour le Développement - IRD
Other partners > Université de Montpellier 2 (FRANCE)
French research institutions > Centre National de la Recherche Scientifique - CNRS
Other partners > Montpellier SupAgro (FRANCE)
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Deposited By:Dabernat Séverine

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