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The use of ethylene-suppressed lines to assess differential sensitivity to ethylene of the various ripening pathways in Cantaloupe melons

Flores, Francisco B. and Ben Amor, Mohamed and Pech, Jean-Claude and Bouzayen, Mondher and Latché, Alain and Romojaro, Felix The use of ethylene-suppressed lines to assess differential sensitivity to ethylene of the various ripening pathways in Cantaloupe melons. (2001) Physiologia Plantarum, vol. 1 (n° 1). pp. 128-133. ISSN 0031-9317

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Official URL: http://dx.doi.org/10.1034/j.1399-3054.2001.1130117.x

Abstract

Physiological characterization of ethylene-suppressed Cantaloupe Charentais melons (Cucumis melo var. cantalupensis Naud cv. Védrantais) revealed that some ripening-associated events, like degreening of the rind and cell separation in the peduncular abscission zone, are totally dependent on ethylene. By contrast, some other ripening events, like softening and membrane deterioration, depend only partially on ethylene and display some ethylene-independent components. Application of increasing levels of exogenous ethylene on these antisense 1-aminocyclopropane-1-carboxylic acid oxidase fruits enabled the determination of the gradual sensitivity of various ripening pathways to the hormone. The threshold level of ethylene capable of physiological activity varied from 1 ppm for degreening of the rind to 2.5 ppm for softening, membrane deterioration and cell separation in the peduncular abscission zone. Up to a saturating dose of 5 ppm, the extent of rind degreening was proportionally related to the level of applied ethylene. The saturating levels of ethylene for flesh softening (2.5 ppm) and for membrane deterioration and cell separation in the peduncular abscission zone (5 ppm) were much lower than the internal ethylene found at the climacteric peak of wild-type fruit (over 100 ppm). The cessation of ethylene treatment resulted in a complete arrest of the rind degreening and peduncular cell separation indicating that both ripening pathways are completely dependent on ethylene. On the contrary, softening and membrane deterioration, though significantly slowed upon removal of ethylene treatment, continued to proceed in the absence of the hormone, thereby unmasking the ethylene-independent component of softening and membrane deterioration. The presence of ethylene-independent components in the regulation of individual pathways indicates that the ripening of climacteric fruit involves a substantial portion of non-climacteric regulation

Item Type:Article
Additional Information:Thanks to Blackwell Publishing editor. The definitive version is available at www.blackwell-synergy.com The original PDF of the article can be found at Physiologia Plantarum : http://www.blackwellpublishing.com/journal.asp?ref=0031-9317
Audience (journal):International peer-reviewed journal
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Institution:Other partners > Consejo Superior de Investigaciones Científicas - CSIC (SPAIN)
French research institutions > Institut National de la Recherche Agronomique - INRA
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
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Deposited By:Dabernat Séverine

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