OATAO - Open Archive Toulouse Archive Ouverte Open Access Week

Biological effects of double-walled carbon nanotubes on the innate immune system: An in vitro study on THP-1 human monocytes

Dekali, Samir and Bachelet, Christine and Maunoir-Regimbal, Séverine and Flahaut, Emmanuel and Debouzy, Jean-Claude and Crouzier, David Biological effects of double-walled carbon nanotubes on the innate immune system: An in vitro study on THP-1 human monocytes. (2016) Toxicology, 365. 1-8. ISSN 0300-483X

[img]
Preview
(Document in English)

PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
458kB

Official URL: http://dx.doi.org/10.1016/j.tox.2016.07.019

Abstract

DWCNTs have numerous industrial and biomedical applications and several studies reported that they could act as immunomodulator systems. The immune system is the first line of defence of the human body when exposed to particulate matter. In order to investigate DWCNTs’ role on innate immunity, we used THP-1 monocytic cells for the purpose of this study. We showed that DWCNTs were not cytotoxic until 6 h, 24 h, 48 h and 72 h of incubation with THP-1 monocytic cells (concentrations tested from 10 to 50 μg/mL). From 6 h to 72 h of incubation of THP-1 cells with DWCNTs, we measured a significant increase of the baseline cell index using xCELLigence® technology showing cell adhesion. After 24 h of exposure, DWCNTs agglomerates were localized in THP-1 monocyte cytoplasm and cell adhesion was observed simultaneously with a significant increase in the expression of CD11b and CD14 cell surface proteins. Pro-inflammatory cytokine secretion (IL-1β, IL-6, IL-8, TNF-α and IL-10) was also measured in supernatants after 6 h or 24 h of exposure to DWCNTs. This pro-inflammatory response was increased in THP-1 monocytic cells pre-treated with LPS. Altogether, our data indicate that DWCNTs induce an increased pro-inflammatory response of THP-1 monocytes and seem to modulate cell surface protein expression confirming that DWCNTs could act as stimulators of innate immunity.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Toxicology website : http://www.sciencedirect.com/science/journal/0300483X
HAL Id:hal-01486943
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Laboratory name:
Statistics:download
Deposited By: cirimat webmestre
Deposited On:10 Mar 2017 15:03

Repository Staff Only: item control page