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Complement activation by carbon nanotubes and its influence on the phagocytosis and cytokine response by macrophages

Pondman, Kirsten M. and Sobik, Martin and Nayak, Annapurna and Tsolaki, Anthony G. and Jäkel, Anne and Flahaut, Emmanuel and Hampel, Silke and ten Haken, Bennie and Sim, Robert B. and Kishore, Uday Complement activation by carbon nanotubes and its influence on the phagocytosis and cytokine response by macrophages. (2014) Nanomedicine: Nanotechnology, Biology and Medicine, 10 (6). 1287-1299. ISSN 1549-9634

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Official URL: http://dx.doi.org/10.1016/j.nano.2014.02.010

Abstract

Carbon nanotubes (CNTs) have promised a range of applications in biomedicine. Although influenced by the dispersants used, CNTs are recognized by the innate immune system, predominantly by the classical pathway of the complement system. Here, we confirm that complement activation by the CNT used continues up to C3 and C5, indicating that the entire complement system is activated including the formation of membrane-attack complexes. Using recombinant forms of the globular regions of human C1q (gC1q) as inhibitors of CNT-mediated classical pathway activation, we showthatC1q, the first recognition subcomponent of the classical pathway, bindsCNTs via the gC1q domain. Complement opsonisation of CNTs significantly enhances their uptake by U937 cells, with concomitant downregulation of pro-inflammatory cytokines and upregulation of anti-inflammatory cytokines in both U937 cells and human monocytes. We propose that CNT-mediated complement activation may cause recruitment of cellular infiltration, followed by phagocytosis without inducing a pro-inflammatory immune response.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at http://www.sciencedirect.com/science/article/pii/S1549963414001129
HAL Id:hal-01451043
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Other partners > Kingston University (UNITED KINGDOM)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Brunel University (UNITED KINGDOM)
Other partners > Leibniz-Institut für Festkörper- und Werkstoffforschung (GERMANY)
Other partners > University of Oxford (UNITED KINGDOM)
Other partners > University of Twente (NETHERLANDS)
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Deposited By: cirimat webmestre
Deposited On:31 Jan 2017 14:48

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