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Combinatorial MAPLE deposition of antimicrobial orthopedic maps fabricated from chitosan and biomimetic apatite powders

Visan, Anita Ioana and Stan, George E. and Ristoscu, Carmen and Popescu-Pelin, Gianina and Sopronyi, Mihai and Besleaga, Cristina and Luculescu, Catalin and Chifiriuc, Mariana Carmen and Hussien, M.D. and Marsan, Olivier and Kergourlay, Emmanuelle and Grossin, David and Brouillet, Fabien and Mihailescu, Ion N. Combinatorial MAPLE deposition of antimicrobial orthopedic maps fabricated from chitosan and biomimetic apatite powders. (2016) International Journal of Pharmaceutics, 511 (1). 505-515. ISSN 0378-5173

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Official URL: http://dx.doi.org/10.1016/j.ijpharm.2016.07.015

Abstract

Chitosan/biomimetic apatite thin films were grown in mild conditions of temperature and pressure by Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates. Compositional gradients were obtained by simultaneous laser vaporization of the two distinct material targets. A KrF* excimer (λ=248nm, τFWHM=25ns) laser source was used in all experiments. The nature and surface composition of deposited materials and the spatial distribution of constituents were studied by SEM, EDS, AFM, GIXRD, FTIR, micro-Raman, and XPS. The antimicrobial efficiency of the chitosan/biomimetic apatite layers against Staphylococcus aureus and Escherichia coli strains was interrogated by viable cell count assay. The obtained thin films were XRD amorphous and exhibited a morphology characteristic to the laser deposited structures composed of nanometric round shaped grains. The surface roughness has progressively increased with chitosan concentration. FTIR, EDS and XPS analyses indicated that the composition of the BmAp-CHT C-MAPLE composite films gradually modified from pure apatite to chitosan. The bioevaluation tests indicated that S. aureus biofilm is more susceptible to the action of chitosan-rich areas of the films, whilst the E. coli biofilm proved more sensible to areas containing less chitosan. The best compromise should therefore go, in our opinion, to zones with intermediate-to-high chitosan concentration which can assure a large spectrum of antimicrobial protection concomitantly with a significant enhancement of osseointegration, favored by the presence of biomimetic hydroxyapatite.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at http://www.sciencedirect.com/science/article/pii/S0378517316306457
HAL Id:hal-01445327
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > University of Bucharest (ROMANIA)
Other partners > National Institute for Lasers, Plasma, and Radiation Physics - INFLPR (ROMANIA)
Other partners > National Institute for Materials Physics - NIMP (ROMANIA)
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Deposited By: Christophe DROUET
Deposited On:24 Jan 2017 16:24

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