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Adsorption of nucleotides on biomimetic apatite: the case of adenosine 5′ monophosphate (AMP)

Hammami, Khaled and El Feki, Hafed and Marsan, Olivier and Drouet, Christophe Adsorption of nucleotides on biomimetic apatite: the case of adenosine 5′ monophosphate (AMP). (2015) Applied Surface Science, 353. 165-172. ISSN 0169-4332

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Official URL: http://dx.doi.org/10.1016/j.apsusc.2015.06.068

Abstract

This work investigates the interaction between the nucleotide adenosine 5′ monophosphate molecule (AMP) and a biomimetic nanocrystalline carbonated apatite as a model for bone mineral. The analogy of the apatite phase used in this work with biological apatite was first pointed out by complementary techniques. AMP adsorption isotherms were then investigated. Obtained data were fitted to a Sips isotherm with an exponent greater than one suggesting positive cooperativity among adsorbed molecules. The data were compared to a previous study relative to the adsorption of another nucleotide, cytidine monophosphate (CMP) onto a similar substrate, evidencing some effect of the chemical nature of the nucleic base. An enhanced adsorption was observed under acidic (pH 6) conditions as opposed to pH 7.4, which parallels the case of DNA adsorption on biomimetic apatite. An estimated standard Gibbs free energy associated to the adsorption process (DG◦ads ∼ −22 kJ/mol) intermediate between “physisorption” and “chemisorption” was found. The analysis of the solids after adsorption pointed to the preservation of the main characteristics of the apatite substrate but shifts or enhancements of Raman bands attributed to AMP showed the existence of chemical interactions involving both the phosphate and adenine parts of AMP. This contribution adds to the works conducted in view of better understanding the interaction of DNA/RNA and their constitutive nucleotides and the surface of biomimetic apatites. It could prove helpful in disciplines such as bone diagenesis (DNA/apatite interface in aged bones) or nanomedicine (setup of DNA or RNA loaded apatite systems). Also, the adsorption of nucleic acids on minerals like apatites could have played a role in the preservation of such biomolecules in the varying conditions known to exist at the origin of life on Earth, underlining the importance of dedicated adsorption studies.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at : https://www.journals.elsevier.com/applied-surface-science/
HAL Id:hal-01451898
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Université de Sfax (TUNISIA)
Laboratory name:
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Deposited By: Christophe DROUET
Deposited On:17 Jan 2017 12:37

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