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Facile and selective covalent grafting of an RGD-peptide to electrospun scaffolds improves HUVEC adhesion

Dettin, Monica and Zamuner, Annj and Roso, Martina and Iucci, Giovanna and Samouillan, Valérie and Danesin, Roberta and Modesti, Michele and Conconi, Maria Teresa Facile and selective covalent grafting of an RGD-peptide to electrospun scaffolds improves HUVEC adhesion. (2015) Journal of Peptide Science, 21 (10). 786-795. ISSN 1075-2617

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Official URL: http://dx.doi.org/10.1002/psc.2808

Abstract

The development of a biomimetic surface able to promote endothelialization is fundamental in the search for blood vessel substitutes that prevent the formation of thrombi or hyperplasia. This study aims at investigating the effect of functionalization of poly-ε-caprolactone or poly(L-lactic acid-co-ɛ-caprolactone) electrospun scaffolds with a photoreactive adhesive peptide. The designed peptide sequence contains four Gly-Arg-Gly-Asp-Ser-Pro motifs per chain and a p-azido-Phe residue at each terminus. Different peptide densities on the scaffold surface were obtained by simply modifying the peptide concentration used in pretreatment of the scaffold before UV irradiation. Scaffolds of poly-ε-caprolactone embeddedwith adhesive peptideswere produced to assess the importance of peptide covalent grafting. Our results show that the scaffolds functionalized with photoreactive peptides enhance adhesion at 24h with a dosedependent effect and control the proliferation of human umbilical vein endothelial cells, whereas the inclusion of adhesive peptide in the electrospun matrices by embedding does not give satisfactory results.

Item Type:Article
Additional Information:Thanks to Wiley editor. The definitive version is available at http://onlinelibrary.wiley.com The original PDF of the article can be found at Thermochimica Acta website : http://onlinelibrary.wiley.com/doi/10.1002/psc.2808/abstract
HAL Id:hal-01345999
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Università degli Studi Roma Tre (ITALY)
Other partners > Università degli Studi di Padova (ITALY)
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Deposited By: cirimat webmestre
Deposited On:22 Jun 2016 12:15

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