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Ethylene response factors are controlled by multiple harvesting stresses in hevea brasiliensis

Putranto, Riza-Arief and Duan, Cuifang and Kuswanhadi, Kuswanhadi and Chaidamsari, Tetty and Rio, Maryannick and Piyatrakul, Piyanuch and Herlinawati, Eva and Pirrello, Julien and Dessailly, Florence and Leclercq, Julie and Bonnot, François and Tang, Chaorong and Hu, Songnian and Montoro, Pascal Ethylene response factors are controlled by multiple harvesting stresses in hevea brasiliensis. (2015) PLoS ONE, 10 (4). e0123618. ISSN 1932-6203

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Official URL: http://dx.doi.org/10.1371/journal.pone.0123618

Abstract

Tolerance of recurrent mechanical wounding and exogenous ethylene is a feature of the rubber tree. Latex harvesting involves tapping of the tree bark and ethephon is applied to increase latex flow. Ethylene is an essential element in controlling latex production. The ethylene signalling pathway leads to the activation of Ethylene Response Factor (ERF) transcription factors. This family has been identified in Hevea brasiliensis. This study set out to understand the regulation of ERF genes during latex harvesting in relation to abiotic stress and hormonal treatments. Analyses of the relative transcript abundance were carried out for 35 HbERF genes in latex, in bark from mature trees and in leaves from juvenile plants under multiple abiotic stresses. Twenty-one HbERF genes were regulated by harvesting stress in laticifers, revealing an overrepresentation of genes in group IX. Transcripts of three HbERF-IX genes from HbERF-IXc4, HbERF-IXc5 and HbERF-IXc6 were dramatically accumulated by combining wounding, methyl jasmonate and ethylene treatments. When an ethylene inhibitor was used, the transcript accumulation for these three genes was halted, showing ethylene-dependent induction. Subcellular localization and transactivation experiments confirmed that several members of HbERF-IX are activator-type transcription factors. This study suggested that latex harvesting induces mechanisms developed for the response to abiotic stress. These mechanisms probably depend on various hormonal signalling pathways. Severalmembers of HbERF-IX could be essential integrators of complex hormonal signalling pathways in Hevea.

Item Type:Article
Additional Information:Thanks to Public Library of Science editor. The definitive version is available at http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0123618
HAL Id:hal-01494937
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > Chinese Academy of Tropical Agricultural Sciences - CATAS (CHINA)
French research institutions > Centre de Coopération Internationale en Recherche Agronomique - CIRAD (FRANCE)
Other partners > Chinese Academy of Sciences (CHINA)
Other partners > Indonesian Biotechnology Research Institute for Estate Crops - IBRIEC (INDONESIA)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Other partners > Indonesian Rubber Research Institute - IRRI (INDONESIA)
Other partners > Montpellier SupAgro (FRANCE)
Other partners > Rubber Research Institute Of Thailand - RRIT (THAILAND)
Laboratory name:
Funders:
info:The French Government (BGF scholarship) provided a 18-month scholarship to Mr. Riza-Arief Putranto - info:The Chinese Scholarship Council provided a 3-year scholarship to Mrs. Cuifang Duan - info:The Institut Français du Caoutchouc (IFC), and the Michelin, Socfinco and Société Internationale de PLantations d'Hévéas (SIPH)companies provided a 18-month scholarship to Mr. Riza-Arief Putranto, and funded all bench fees for experiments - info:CATAS and the Beijing Institute of Genomics provided access to the Hevea genome sequence
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Deposited By: Brigitte LAFFORGUE
Deposited On:24 Mar 2017 10:52

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