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Dynamics of bacterial assemblages and removal of polycyclic aromatic hydrocarbons in oil-contaminated coastal marine sediments subjected to contrasted oxygen regimes

Militon, Cécile and Jezequel, Ronan and Gilbert, Franck and Corsellis, Yannick and Sylvi, Léa and Cravo-Laureau, Cristiana and Duran, Robert and Cuny, Philippe Dynamics of bacterial assemblages and removal of polycyclic aromatic hydrocarbons in oil-contaminated coastal marine sediments subjected to contrasted oxygen regimes. (2015) Environmental Science and Pollution Research, 22 (20). 15260-15272. ISSN 0944-1344

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Official URL: http://dx.doi.org/10.1007/s11356-015-4510-y

Abstract

To study the impact of oxygen regimes on the removal of polycylic aromatic hydrocarbons (PAHs) in oil-spill-affected coastal marine sediments, we used a thin-layer incubation method to ensure that the incubated sediment was fully oxic, anoxic, or was influenced by oxic-anoxic switches without sediment stirring. Hydrocarbon content and microbial assemblages were followed during 60 days to determine PAH degradation kinetics and microbial community dynamics according to the oxygenation regimes. The highest PAH removal, with 69 % reduction, was obtained at the end of the experiment under oxic conditions, whereas weaker removals were obtained under oscillating and anoxic conditions (18 and 12 %, respectively). Bacterial community structure during the experiment was determined using a dual 16S rRNA genes/16S rRNA transcripts approach, allowing the characterization of metabolically active bacteria responsible for the functioning of the bacterial community in the contaminated sediment. The shift of the metabolically active bacterial communities showed that the selection of first responders belonged to Pseudomonas spp. and Labrenzia sp. and included an unidentified Deltaproteobacteria—irrespective of the oxygen regime—followed by the selection of late responders adapted to the oxygen regime. A novel unaffiliated phylotype (B38) was highly active during the last stage of the experiment, at which time, the low-molecular-weight (LMW) PAH biodegradation rates were significant for permanent oxic- and oxygen-oscillating conditions, suggesting that this novel phylotype plays an active role during the restoration phase of the studied ecosystem.

Item Type:Article
Additional Information:Thanks to Springer editor. The definitive version is available at http://link.springer.com/ The original PDF of the article can be found at Environmental Science and Pollution Research website : http://link.springer.com/journal/11356
HAL Id:hal-01233392
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > Aix-Marseille Université - AMU (FRANCE)
Other partners > Centre de Documentation, de Recherche et d'Expérimentations sur les pollutions accidentelles des eaux - CEDRE (FRANCE)
French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
French research institutions > Institut de Recherche pour le Développement - IRD (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Université du Sud Toulon-Var - USTV (FRANCE)
Other partners > Université de Pau et des Pays de l'Adour - UPPA (FRANCE)
Laboratory name:
Funders:
ANR
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Deposited By: Franck Gilbert
Deposited On:25 Nov 2015 08:50

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