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Sl-ERF2, a Tomato Ethylene Response Factor Involved in Ethylene Response and Seed Germination

Pirrello, Julien and Jaimes-Miranda, Fabiola and Sanchez-Ballesta, Maria Teresa and Tournier, Barthélémy and Khalil-Ahmad, Qaiser and Regad, Farid and Latché, Alain and Pech, Jean-Claude and Bouzayen, Mondher Sl-ERF2, a Tomato Ethylene Response Factor Involved in Ethylene Response and Seed Germination. (2006) Plant & Cell Physiology, vol. 4 (n° 9). pp. 1195-1205. ISSN 0032-0781

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Official URL: http://dx.doi.org/10.1093/pcp/pcj084

Abstract

Ethylene response factors (ERFs) are plant transcriptional regulators mediating ethylene-dependent gene expression via binding to the GCC motif found in the promoter region of ethylene-regulated genes. We report here on the structural and functional characterization of the tomato Sl-ERF2 gene that belongs to a distinct class of the large ERF gene family. Both spliced and unspliced versions of Sl-ERF2 transcripts were amplified from RNA samples and the search in the public tomato expressed sequence tag (EST) database confirmed the existence of the two transcript species in a number of cDNA libraries. The unspliced transcript contains two open reading frames yielding two hypothetical proteins, a small highly truncated version lacking the APETALA2 domain and a bigger protein lacking the N-terminal MCGGAAII/L consensus peptide specific to ERF members from subfamily IV. Nevertheless, functional Sl-ERF2 protein may only derive from spliced transcripts since, depending on the tissue, the level of the spliced transcript is much higher than that of the unspliced transcript. Sl-ERF2 is expressed in all plant tissues tested, though its transcript accumulates preferentially in germinating seeds and ripening fruit. Overexpression of the Sl-ERF2 gene in transgenic tomato lines results in premature seed germination and enhanced hook formation of darkgrown seedlings, which is indicative of increased ethylene sensitivity. The expression of the mannanase2 gene is upregulated in Sl-ERF2-overexpressing seeds, suggesting that Sl-ERF2 stimulates seed germination through the induction of the mannanase2 gene. It is noteworthy that the exaggerated hook phenotype is abolished when ethylene perception is blocked, strongly suggesting that Sl-ERF2 requires other ethylene-dependent components to impact the hook formation process.

Item Type:Article
Additional Information:Thanks to Oxford University Press The original PDF of the article can be found at Plant & Cell Physiology website : http://pcp.oxfordjournals.org/cgi/content/abstract/47/9/1195
Audience (journal):International peer-reviewed journal
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Institution:French research institutions > Institut National de la Recherche Agronomique - INRA
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
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Deposited By: Dabernat Séverine
Deposited On:03 Dec 2008 08:54

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