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Differential regulation of ACC synthase genes in cold-dependent and -independent ripening in pear fruit

El-Sharkawy, Islam and Jones, Brian and Gentzbittel, Laurent and Lelièvre, Jean-Marc and Pech, Jean-Claude and Latché, Alain Differential regulation of ACC synthase genes in cold-dependent and -independent ripening in pear fruit. (2004) Plant Cell & Environment, vol. 2 (n° 10). pp. 1197-1210. ISSN 0140-7791

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Official URL: http://dx.doi.org/10.1111/j.1365-3040.2004.01218.x

Abstract

Late pear cultivars such as Passe-Crassane (PC) require a long chilling treatment before they are capable of ripening. Early cultivars such as Old-Home (OH) have no cold prerequisite. The regulation of 1-aminocyclopropane-1-carboxylic acid synthase (ACS) genes was studied in OH, PC and in OH x PC hybrids in order to determine the role of this gene family in the cold requirement. Of the seven Pc-ACS cDNAs isolated, four (Pc-ACSla/b and Pc-ACS2a/b) showed differential expression associated with the cold requirement. Pc-ACS1a transcripts accumulated throughout the cold treatment and, with Pc-ACS2a, during ripening of cold-dependent cultivars. Pc-ACS1b and Pc-ACS2b were detected only during ripening of cold-independent genotypes. Furthermore, Pc-ACS2a transcript accumulation was negatively regulated by ethylene, whereas Pc-ACS2b was positively regulated by the hormone. Pc-ACS3, 4 and 5 transcript accumulation was similar in all genotypes. Genetic analyses of OH, PC, and 22 OH x PC progenies demonstrated that late, cold-dependent cultivars were homozygous for Pc-ACS1a and 2a whereas early, cold-independent cultivars were heterozygous for Pc-ACS1(a/b) and homozygous for Pc-ACS2b. A model is presented in which differences in Pc-ACS alleles and gene expression between cold- and non-cold-requiring pears are critical in determining the ripening behaviour of the cultivars.

Item Type:Article
Additional Information:Thanks to Blackwell Publishing editor.The definitive version is available at www.blackwell-synergy.com. The original PDF of the article can be found at Plant Cell & Environment website : http://www3.interscience.wiley.com/journal/118769037/abstract
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut National de la Recherche Agronomique - INRA
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Other partners > University of Melbourne (AUSTRALIA)
French research institutions > Centre National de la Recherche Scientifique - CNRS
Laboratory name:
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Deposited By: Dabernat Séverine

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