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Auxin Perception Is Required for Arbuscule Development in Arbuscular Mycorrhizal Symbiosis

Etemadi-Shalamzari, Mohammad and Gutjahr, Caroline and Couzigou, Jean-Malo and Zouine, Mohamed and Lauressergues, Dominique and Timmers, Antonius and Audran-Delalande, Corinne and Bouzayen, Mondher and Bécard, Guillaume and Combier, Jean-Philippe Auxin Perception Is Required for Arbuscule Development in Arbuscular Mycorrhizal Symbiosis. (2014) Plant Physiology, vol. 166 (n° 1). pp. 281-292. ISSN 0032-0889

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Official URL: http://dx.doi.org/10.1104/pp.114.246595

Abstract

Most land plant species live in symbiosis with arbuscular mycorrhizal fungi. These fungi differentiate essential functional structures called arbuscules in root cortical cells from which mineral nutrients are released to the plant. We investigated the role of microRNA393 (miR393), an miRNA that targets several auxin receptors, in arbuscular mycorrhizal root colonization. Expression of the precursors of the miR393 was down-regulated during mycorrhization in three different plant species: Solanum lycopersicum, Medicago truncatula, and Oryza sativa. Treatment of S. lycopersicum, M. truncatula, and O. sativa roots with concentrations of synthetic auxin analogs that did not affect root development stimulated mycorrhization, particularly arbuscule formation. DR5-GUS, a reporter for auxin response, was preferentially expressed in root cells containing arbuscules. Finally, overexpression of miR393 in root tissues resulted in down-regulation of auxin receptor genes (transport inhibitor response1 and auxin-related F box) and underdeveloped arbuscules in all three plant species. These results support the conclusion that miR393 is a negative regulator of arbuscule formation by hampering auxin perception in arbuscule-containing cells.

Item Type:Article
Additional Information:Thanks to American Society of Plant Biologists editor. The definitive version is available at http://www.plantphysiol.org/ The original PDF of the article can be found at Plant Physiology website : http://www.plantphysiol.org/content/166/1/281.full
HAL Id:hal-01518866
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Ludwig Maximilians Universiät München - LMU (GERMANY)
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Deposited By: Brigitte LAFFORGUE
Deposited On:05 May 2017 13:11

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