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Characterization of three members of the Arabidopsis carotenoid cleavage dioxygenase family demonstrates the divergent roles of this multifunctional enzyme family

Auldridge, Michele E. and Block, Anna and Vogel, Jonathan T. and Dabney-Smith, Carole and Mila, Isabelle and Bouzayen, Mondher and Magallanes-Lundback, Maria and DellaPenna, Dean and McCarty, Donald R. and Klee, Harry J. Characterization of three members of the Arabidopsis carotenoid cleavage dioxygenase family demonstrates the divergent roles of this multifunctional enzyme family. (2006) The Plant Journal, vol. 4 (n° 6). pp. 968-981. ISSN 0960-7412

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Official URL: http://dx.doi.org/10.1111/j.1365-313X.2006.02666.x

Abstract

Arabidopsis thaliana has nine genes that constitute a family of putative carotenoid cleavage dioxygenases (CCDs). While five members of the family are believed to be involved in synthesis of the phytohormone abscisic acid, the functions of the other four enzymes are less clear. Recently two of the enzymes, CCD7/MAX3 and CCD8/MAX4, have been implicated in synthesis of a novel apocarotenoid hormone that controls lateral shoot growth. Here, we report on the molecular and genetic interactions between CCD1, CCD7/MAX3 and CCD8/ MAX4. CCD1 distinguishes itself from other reported CCDs as being the only member not targeted to the plastid. Unlike ccd7/max3 and ccd8/max4, both characterized as having highly branched phenotypes, ccd1 loss-of-function mutants are indistinguishable from wild-type plants. Thus, even though CCD1 has similar enzymatic activity to CCD7/MAX3, it does not have a role in synthesis of the lateral shoot growth inhibitor. Rather, it may have a role in synthesis of apocarotenoid flavor and aroma volatiles, especially in maturing seeds where loss of function leads to significantly higher carotenoid levels.

Item Type:Article
Additional Information:The original PDF of the article can be found at The Plant Journal website : http://www3.interscience.wiley.com/journal/118565007/abstract
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut National de la Recherche Agronomique - INRA
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Other partners > University of Florida (USA)
Other partners > Michigan State University - MSU (USA)
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Deposited By: Dabernat Séverine
Deposited On:12 Nov 2008 10:36

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