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ESTs, cDNA microarrays, and gene expression profiling : tools for dissecting plant physiology and development

Alba, Rob and Fei, Zhangjun and Payton, Paxton and Liu, Yang and Moore, Shanna L. and Debbie, Paul and Cohn, Jonathan and D’Ascenzo, Mark and Gordon, Jeffrey S. and Rose, Jocelyn K. C. and Martin, Gregory and Tanksley, Steven D. and Bouzayen, Mondher and Jahn, Molly M. and Giovannoni, Jim ESTs, cDNA microarrays, and gene expression profiling : tools for dissecting plant physiology and development. (2004) The Plant Journal, vol. 3 (n° 5). pp. 697-714. ISSN 0960-7412

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Official URL: http://dx.doi.org/10.1111/j.1365-313X.2004.02178.x

Abstract

Gene expression profiling holds tremendous promise for dissecting the regulatory mechanisms and transcriptional networks that underlie biological processes. Here we provide details of approaches used by others and ourselves for gene expression profiling in plants with emphasis on cDNA microarrays and discussion of both experimental design and downstream analysis. We focus on methods and techniques emphasizing fabrication of cDNA microarrays, fluorescent labeling, cDNA hybridization, experimental design, and data processing. We include specific examples that demonstrate how this technology can be used to further our understanding of plant physiology and development (specifically fruit development and ripening) and for comparative genomics by comparing transcriptome activity in tomato and pepper fruit.

Item Type:Article
Additional Information:The original PDF of the article can be found at The Plant Journal website : http://www3.interscience.wiley.com/journal/118794011/abstract
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut National de la Recherche Agronomique - INRA
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Other partners > Cornell University (USA)
Laboratory name:
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Deposited By: Dabernat Séverine
Deposited On:12 Nov 2008 10:18

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