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Comparison of four methods for quantification of biofilms in biphasic cultures

Joannis-Cassan, Claire and Délia, Marie-Line and Riba, Jean-Pierre Comparison of four methods for quantification of biofilms in biphasic cultures. (1998) Biotechnology Techniques, vol. 12 (n° 10). pp. 777-782. ISSN 1573-6784

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Official URL: http://dx.doi.org/10.1023/A:1008835811731

Abstract

Three methods for determining the total biofilm amount in biphasic cultures have been compared: dry weight by filtration after solvent treatment, optical density with a biomass probe and protein content. The activity of the biofilm was estimated through mineral nitrogen consumption. Calculation of the coefficients of variation shows that these parameters could be used to characterise such a biofilm. The optical density by biomass probe was the most reliable one (repeatability <0.5%) to quantify total biofilm and a linear relation was verified against dry weight.

Item Type:Article
Additional Information:Thanks to SpringerLink editor. The definitive version is available at http://www.springerlink.com The original PDF of the article can be found at Biotechnology Techniques website : http://www.springerlink.com/content/100137
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution: Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
French research institutions > Centre National de la Recherche Scientifique - CNRS
Laboratory name:
Laboratoire de Génie Chimique - LGC (Toulouse, France) - Bioprocédés et systèmes microbiens (BioSyM)
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Deposited By:Hélène Dubernard

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