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Amplification and diversity analysis of ketosynthase domains of putative polyketide synthase genes in Aspergillus ochraceus and Aspergillus carbonarius producers of ochratoxin A

Atoui, Ali and Phong Dao, Huy and Mathieu, Florence and Lebrihi, Ahmed Amplification and diversity analysis of ketosynthase domains of putative polyketide synthase genes in Aspergillus ochraceus and Aspergillus carbonarius producers of ochratoxin A. (2006) Molecular Nutrition & Food Research, vol. 5 (n° 6). pp. 488-493. ISSN 1613-4133

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Official URL: http://dx.doi.org/10.1002/mnfr.200500165

Abstract

The diversity of polyketide synthase (PKS) genes in Aspergillus ochraceus NRRL 3174 and Aspergillus carbonarius 2Mu134 has been investigated using different primer pairs previously developed for the ketosynthase (KS) domain of fungal PKSs. Nine different KS domain sequences in A. ochraceus NRRL 3174 as well as five different KS domain sequences in A. carbonarius 2Mu134 have been identified. The identified KS fragments were distributed in five different clusters on the phylogenetic tree, indicating that they most probably represent PKSs responsible for different functions.

Item Type:Article
Additional Information:Thanks to Wiley Interscience editor. The definitive version is available at http://www3.interscience.wiley.com The original PDF of the article can be found at http://www3.interscience.wiley.com/journal/117935711 website : http://www.sciencedirect.com/science/journal/00406031
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution: Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
French research institutions > Centre National de la Recherche Scientifique - CNRS
Laboratory name:
Laboratoire de Génie Chimique - LGC (Toulouse, France) - Bioprocédés et systèmes microbiens (BioSyM)
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Deposited By:Hélène Dubernard

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