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The Auxin Sl-IAA17 Transcriptional Repressor Controls Fruit Size Via the Regulation of Endoreduplication-Related Cell Expansion.

Su, Liyan and Bassa, Carole and Audran-Delalande, Corinne and Mila, Isabelle and Cheniclet, Catherine and Chevalier, Christian and Bouzayen, Mondher and Roustan, Jean-Paul and Chervin, Christian The Auxin Sl-IAA17 Transcriptional Repressor Controls Fruit Size Via the Regulation of Endoreduplication-Related Cell Expansion. (2014) Plant and Cell Physiology, 26 (11). 1969-1976. ISSN 0032-0781

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Abstract

Auxin is known to regulate cell division and cell elongation, thus controlling plant growth and development. Part of the auxin signaling pathway depends on the fine-tuned degradation of the auxin/indole acetic acid (Aux/IAA) transcriptional repressors. Recent evidence indicates that Aux/IAA proteins play a role in fruit development in tomato (Solanum lycopersicum Mill.), a model species for fleshy fruit development. We report here on the functional characterization of Sl-IAA17 during tomato fruit development. Silencing of Sl-IAA17 by an RNA interference (RNAi) strategy resulted in the production of larger fruit than the wild type. Histological analyses of the fruit organ and tissues demonstrated that this phenotype was associated with a thicker pericarp, rather than larger locules and/or a larger number of seeds. Microscopic analysis demonstrated that the higher pericarp thickness in Sl-IAA17 RNAi fruits was not due to a larger number of cells, but to the increase in cell size. Finally, we observed that the cell expansion in the transgenic fruits is tightly coupled with higher ploidy levels than in the wild type, suggesting a stimulation of the endoreduplication process. In conclusion, this work provides new insights into the function of the Aux/IAA pathway in fleshy fruit development, especially fruit size and cell size determination in tomato. Su L1, Bassa C1, Audran C1, Mila I1, Cheniclet C2, Chevalier C2, Bouzayen M1, Roustan JP1, Chervin C3.

Item Type:Article
Additional Information:Thanks to Oxford University Press editor. The definitive version is available at
HAL Id:hal-01335934
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Other partners > Université de Bordeaux (FRANCE)
Laboratory name:
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Deposited By: Christian CHERVIN
Deposited On:16 Jan 2015 15:44

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