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DNA adducts in fish following an oil spill exposure

Amat, A and Burgeot, T and Castegnaro, Marcel and Pfohl-Leszkowicz, Annie DNA adducts in fish following an oil spill exposure. (2006) Environmental Chemistry Letters, vol. 4 (n° 2). pp. 93-99. ISSN 1610-3661

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Official URL: http://dx.doi.org/10.1007/s10311-006-0040-7

Abstract

On 12 December 1999, one third of the load of the Erika tanker, amounting to about 10,000 t crude oil flowed into sea waters close to the French Atlantic Coast. This oil contained polycyclic aromatic compounds (PAC) that are known to be genotoxic. Genotoxic effects induce DNA adducts formation, which can thus be used as pollution biomarkers. Here, we assessed the genotoxic impact of the “Erika” oil spill by DNA adducts detection in the liver of immature fishes (Solea solea) from four locations of the French Brittany coasts. Two months after the spill, a high amount of DNA adducts was found in samples from all locations, amounting to 92–290 DNA adduct per 109 nucleotides. Then total DNA adduct levels decreased to reach about 50 adducts per 109 nucleotides nine months after the spill. In vitro experiments using human cell cultures and fish liver microsomes evidence the genotoxicity of the Erika fuel. They also prove the formation of reactive species able to create DNA adducts. Furthermore, in vitro and in vivo DNA adducts fingerprints are similar, thus confirming that DNA adducts are a result of the oil spill.

Item Type:Article
Additional Information:Thanks to SpringerLink editor. The definitive version is available at http://www.springerlink.com The original PDF of the article can be found at Environmental Chemistry Letters website : http://www.springerlink.com/content/110367
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution: Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
French research institutions > Institut Français de Recherche pour l'Exploitation de la MER - IFREMER
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
French research institutions > Centre National de la Recherche Scientifique - CNRS
Laboratory name:
Laboratoire de Génie Chimique - LGC (Toulouse, France) - Bioprocédés et systèmes microbiens (BioSyM)
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Deposited By:Hélène Dubernard

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