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Enzyme-functionalized biomimetic apatites: concept and perspectives in view of innovative medical approaches

Weber, Christina G. and Mueller, Michaela and Vandecandelaere, Nicolas and Trick, Iris and Burger-Kentischer, Anke and Maucher, Tanja and Drouet, Christophe Enzyme-functionalized biomimetic apatites: concept and perspectives in view of innovative medical approaches. (2014) Journal of Materials Science: Materials in Medicine, 25 (3). 595-606. ISSN 0957-4530

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Official URL: http://dx.doi.org/10.1007/s10856-013-5097-9

Abstract

Biomimetic nanocrystalline calcium-deficient apatite compounds are particularly attractive for the setup of bioactive bone-repair scaffolds due to their high similarity to bone mineral in terms of chemical composition, structural and substructural features. As such, along with the increasingly appealing development of moderate temperature engineered routes for sample processing, they have widened the armamentarium of orthopedic and maxillofacial surgeons in the field of bone tissue engineering. This was made possible by exploiting the exceptional surface reactivity of biomimetic apatite nanocrystals, capable of easily exchanging ions or adsorbing (bio)molecules, thus leading to highly-versatile drug delivery systems. In this contribution we focus on the preparation of hybrid materials combining biomimetic nanocrystalline apatites and enzymes (lysozyme and subtilisin). This paper reports physico-chemical data as well as cytotoxicity evaluations towards Cal-72 osteoblast-like cells and finally antimicrobial assessments towards selected strains of interest in bone surgery. Biomimetic apatite/enzyme hybrids could be prepared in varying buffers. They were found to be non-cytotoxic toward osteoblastic cells and the enzymes retained their biological activity (e.g. bond cleavage or antibacterial properties) despite the immobilization and drying processes. Release properties were also examined. Beyond these illustrative examples, the concept of biomimetic apatites functionalized with enzymes is thus shown to be useable in practice, e.g. for antimicrobial purposes, thus widening possible therapeutic perspectives.

Item Type:Article
Additional Information:Thanks to Springer editor. The definitive version is available at http://link.springer.com/article/10.1007%2Fs10856-013-5097-9
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Institut Fraunhofer-Gesellschaft (GERMANY)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Universität Stuttgart (GERMANY)
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Deposited By: cirimat webmestre
Deposited On:21 Oct 2014 16:39

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