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Simple design of cast myoglobin/polyethyleneimine modified electrodes

Pardo, Aurélie and Basséguy, Régine and Bergel, Alain Simple design of cast myoglobin/polyethyleneimine modified electrodes. (2006) Journal of Applied Electrochemistry, vol. 3 (n° 7). pp. 835-842. ISSN 1572-8838

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Official URL: http://dx.doi.org/10.1007/s10800-006-9146-7

Abstract

Negatively charged myoglobin (at pH values above its isoelectric point) was immobilized with the positively charged polyion polyethyleneimine (PEI) on pyrolytic graphite electrodes. A modified form of the common layer-by-layer technique was proposed, which consisted in forming non-ordered cast polyion films. The modified Mb electrodes obtained by both techniques were compared by cyclic voltammetry. The cast polyion technique gave less reproducible results but enable the immobilization of higher percentages of the total quantity of protein required to prepare the electrodes. The electrochemical properties of the negatively charged myoglobin were determined, and its capability to catalyze the electrochemical reduction of oxygen and the dechlorination of trichloroacetic acid was demonstrated. The mechanisms are discussed, and the modified pathway that has been proposed to take into account the influence of pH in the Mb-catalyzed reduction of oxygen was confirmed here.

Item Type:Article
Additional Information:Thanks to SpringerLink editor. The definitive version is available at http://www.springerlink.com The original PDF of the article can be found at Journal of Applied Electrochemistry website : http://www.springerlink.com/content/100178
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution: Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
French research institutions > Centre National de la Recherche Scientifique - CNRS
Laboratory name:
Laboratoire de Génie Chimique - LGC (Toulouse, France) - Bioprocédés et systèmes microbiens (BioSyM)
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Deposited By:Hélène Dubernard

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