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NADH regenerated using immobilized FDH in a continuously supplied reactor – Application to L-lactate synthesis

Roche, Jérôme and Groenen-Serrano, Karine and Reynes, Olivier and Chauvet, Fabien and Tzedakis, Théodore NADH regenerated using immobilized FDH in a continuously supplied reactor – Application to L-lactate synthesis. (2014) Chemical Engineering Journal, Vol. 239. pp. 216-225. ISSN 1385-8947

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Official URL: http://dx.doi.org/10.1016/j.cej.2013.10.096

Abstract

The present study deals with the design and optimization of a reduced-scale filter press reactor, containing an immobilized layer of formate dehydrogenase between two layers of chitosan, dedicated to the enzymatic synthesis of chiral molecules. Elaboration and optimization of the overall system was carried out to demonstrate the feasibility of the immobilization of formate dehydrogenase in a continuous synthesis process. The results demonstrate that the immobilized FDH keeps half of its enzymatic activity for practically two weeks. In addition, the polymeric matrix allows transfer of NAD+ with relatively high diffusion coefficients (2.1 × 10−11 m2 s−1). Experimental validation of NADH regeneration was achieved for pyruvate reduction to L-lactate. Simulation demonstrated that it is possible to achieve practically quantitative conversion of the NAD+ if the reaction channel reaches a length of half a meter.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Chemical Engineering Journal website : http://www.sciencedirect.com/science/article/pii/S1385894713014253
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS (FRANCE)
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Deposited By: Karine Groenen Serrano
Deposited On:16 Jan 2014 15:23

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