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Head tracking and flagellum tracing for sperm motility analysis

Yang, Hue-Fang and Descombes, Xavier and Prigent, Sylvain and Malandain, Grégoire and Druart, Xavier and Plouraboué, Franck Head tracking and flagellum tracing for sperm motility analysis. (2014) In: ISBI - IEEE International Symposium on Biomedical Imaging, 29 April 2014 - 2 May 2014 (Beijing, China). (Unpublished)

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Abstract

Sperm quality assessment plays an essential role in human fertility and animal breeding. Manual analysis is time-consuming and subject to intra- and inter-observer variability. To automate the analysis process, as well as to offer a means of statistical analysis that may not be achieved by visual inspection, we present a computational framework that tracks the heads and traces the tails for analyzing sperm motility, one of the most important attributes in semen quality evaluation. Our framework consists of 3 modules: head detection, head tracking, and flagellum tracing. The head detection module detects the sperm heads from the image data, and the detected heads are the inputs to the head tracking module for obtaining the head trajectories. Finally, a flagellum tracing algorithm is proposed to obtain the flagellar beat patterns. Our framework aims at providing both the head trajectories and the flagellar beat patterns for quantitatively assessing sperm motility. This distinguishes our work from other existing methods that analyze sperm motility based merely on the head trajectories. We validate our framework using two confocal microscopy image sequences of ram semen samples that were imaged at two different conditions, at which the sperms behave differently. The results show the effectiveness of our framework.

Item Type:Conference or Workshop Item (Paper)
Additional Information:This work is supported by ANR MOTIMO Project
Audience (conference):International conference proceedings
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
French research institutions > Institut National de la Recherche en Informatique et en Automatique - INRIA (FRANCE)
French research institutions > Institut National de la Santé et de la Recherche Médicale - INSERM (FRANCE)
Other partners > Université Nice Sophia Antipolis (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Laboratory name:
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Deposited By: Franck PLOURABOUE
Deposited On:06 Jan 2014 11:09

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