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Chemical Composition and in Vitro Evaluation of the Antioxidant and Antimicrobial Activities of Eucalyptus gillii Essential Oil and Extracts

Ben Hassine, Dorsaf and Abderrabba, Manef and Yvon, Yan and Lebrihi, Ahmed and Mathieu, Florence and Couderc, François and Bouajila, Jalloul Chemical Composition and in Vitro Evaluation of the Antioxidant and Antimicrobial Activities of Eucalyptus gillii Essential Oil and Extracts. (2012) Molecules, vol. 17 (n° 8). pp. 9540-9558. ISSN 1420-3049

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Official URL: http://dx.doi.org/10.3390/molecules17089540

Abstract

In this study, essential oil and various extracts (hexane, petroleum ether, acetone, ethanol, methanol and water) of Eucalyptus gilii were screened for their chemical composition, antimicrobial and antioxidant activities. The essential oil chemical composition was analyzed by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID), respectively. Thirty four compounds were identified, corresponding to 99.5% of the total essential oil. Tannins [104.9-251.3 g catechin equivalent (CE)/Kg dry mass], flavonoids [3.3-34.3 g quercetin equivalent (QE)/Kg dry mass], phenolics [4.7-216.6 g gallic acid equivalent (GAE)/Kg dry mass] and anthocyannins [1.2-45.3 mg cyanidin-3-glucoside equivalent (C3GE)/Kg dry mass] of various extracts were investigated. Free radical scavenging capacity of all samples was determinedt. In the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, the IC50 of essential oil was 163.5 ± 10.7 mg/L and in the 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate (ABTS) assay, it was 94.7 ± 7.1 mg/L. Among the various extracts, the water extract showed the best result (IC50 = 11.4 ± 0.6 mg/L) in the DPPH assay which was comparable to vitamin C (IC50 = 4.4 ± 0.2 mg/L). The antimicrobial activities were evaluated against different bacterial and fungal strains. Gram positive bacteria were found to be more sensitive to the essential oil and extracts than Gram negative ones. Anthocyanins seem to have a major effect on the growth of Bacillus subtilis (R2 = 0.79). A significant antifungal activity was observed against the yeast and fungi. Correlations between chemical composition and antioxidant activities were studied and R2 values were about 0.96 for the effect of phenolics on the DPPH assay.

Item Type:Article
Additional Information:Thanks to MDPI editor. The definitive version is available at http://www.mdpi.com/journal/molecules The original PDF of the article can be found at Molecules website : http://dx.doi.org/10.3390/molecules17089540
HAL Id:hal-00906430
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Other partners > Institut Préparatoire aux Etudes Scientifiques et Techniques - IPEST (TUNISIA)
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS (FRANCE)
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Deposited By: Vincent GERBAUD
Deposited On:15 Nov 2013 15:32

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